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Isolation of Partial Length Plasma Membrane H+-ATPase Gene From Melastoma affine D. Don

Muzunia, Suharsonob, Utut Widyastutib
Plasma membrane H+-ATPase (pma) gene that plays an important role in transportation of nutrient across plasma membrane and pumping H+ go out the cell until pH can be maintained was isolated and sequenced from Melastoma affine D. Don. The cDNA was isolated from leaves using RT-PCR. Sequencing product of the cDNA showed that it is the fragment of plasma membrane H+-ATPase gene, which consists of an 806 base pair and a 268 amino acid, then we called as Mapma fragment. The alignment analysis based on nucleotide sequence in GeneBank using BLAST (Basic Local Alignment Search Tool) program showed that the Mapma fragment had similarity with the plasma membrane H+- ATPase of Sesbania rostrata (AB086374), Juglans regia (AY347715), and Prunus persica (AJ271439). The alignment analysis based on amino acid sequence showed that the Mapma fragment had similarity with Cation-transporting ATPase Sesbania rostrata (Q7Y066), Cation-transporting ATPase Solanum tuberosum (Q43182), Cation-transporting ATPase Juglans regia (Q6V914), and Cation-transporting ATPase Lycopersicon esculentum (Q9SPD5). Key words: Isolation, Melastoma affine D. Don., Mapma fragment, sequencing.

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Diterbitkan pada: Proceedings of the Celebes Intemational Confrence on Diversity of Wallacea's Line (CICWDL) Kendari 8-10 mei 2015, Indonesia ISBN : 978-602-8161-81-7

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